Journal: Cells

Article Title: The Effect of Visfatin on the Functioning of the Porcine Pituitary Gland: An In Vitro Study.

doi: 10.3390/cells12242835

Figure Lengend Snippet: Figure 10. The in vitro effect of visfatin on the apoptosis process in anterior pituitary cells. This study was conducted on anterior pituitary glands harvested from pigs (n = 5) during the mid-luteal phase (the phase of the highest corpus luteum activity throughout the cycle; days 10–12). After isolation, anterior pituitary cells were preincubated for 72 h and then incubated for 24 h with visfatin (VIS) at

Article Snippet: The Swine Annexin V-Fluorescein Apoptosis Assay Kit (ImmunoChemistry Technologies, Davis, CA, USA) was used to examine this process, according to the manufacturer’s instructions.

Techniques: In Vitro, Activity Assay, Isolation, Incubation

Journal: Science Advances

Article Title: A conserved glycan motif induces broadly reactive functional antibodies against the zoonotic pathogen Streptococcus suis

doi: 10.1126/sciadv.adz1854

Figure Lengend Snippet: ( A ) Binding of plant lectins to surface structures of capsule-deficient S. suis strains (ΔCPS). SBA binds to N -acetylgalactosamine (GalNAc) and, to a lesser extent, galactose (Gal); RCA 120 binds to both Gal and GalNAc; sWGA has a special affinity to N -acetylglucosamine (GlcNAc). Data from biological triplicates are presented as mean values ± SD. ( B ) Glycosyl composition analysis by GC-MS of TMS (trimethylsilyl) derivatives of methyl glycosides of S. suis RPS from S10 and 861160 released by mild acid hydrolysis after chemical N -acetylation. ( C ) Plant lectin binding to isolated RPS from S. suis S10 and 861160. Data show technical triplicates (mean values ± SD) and are representative for two independent experiments. ( D and E ) Presence and absence of the most abundant glycosyl linkage residues (D) and phosphate (E) of S. suis RPS from S10 and 861160. Glycosyl linkage residues were analyzed by GC-MS of partially methylated alditol acetate derivatives. Phosphate was determined by malachite green assay following hydrolysis with hydrochloric acid and digestion with alkaline phosphatase. Original data is in tables S2 and S3.

Article Snippet: Subsequently, 12.5 μl of bacterial suspension was incubated in a 96-well V-bottom plate with the same volume of pig serum or fluorescein-labeled lectin (Vector Laboratories, FLK-2100 and FL-1021S-5).

Techniques: Binding Assay, Gas Chromatography-Mass Spectrometry, Isolation, Methylation, Malachite Green Assay